This porcine PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in porcine plasma, serum and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests.
Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, monoclonal anti-porcine PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
More Details:
- Detection Target: PAI-1
- Reactivity: Porcine
- Range: 0.05-20 ng/ml
- Sensitivity: 0.046 ng/ml
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Please note – this product replaces our old product number IPOPAIKT (same product, new number).