This Rabbit Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total rabbit IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. This assay does not distinguish IgG subclasses. For research use only. Strip well format. Reagents for up to 96 tests.
Rabbit IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-rabbit IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit IgG. Color development is directly proportional to the concentration of total IgG in the samples.
More Details:
- Detection Target: IgG
- Reactivity: Rabbit
- Range: 0.1-100 ng/ml
- Sensitivity: 0.051 ng/ml
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Please note – this product replaces our old product number IRBIGGKT (same product, new number).