This rat plasminogen activator inhibitor type 1 (PAI-1) activity assay is intended for the quantitative determination of active PAI-1 in rat plasma and serum. For research use only. Strip well format. Reagents for up to 96 tests.
Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. Unbound PAI-1 samples are washed away and an anti-PAI-1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI-1 present in the samples, is then reacted with the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.
More Details:
- Detection Target: PAI-1
- Reactivity: Rat
- Range: 0.1 50 ng/ml
- Sensitivity: 0.032 ng/ml
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Please note – this product replaces our old product number IRPAIKT (same product, new number).