This rat plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is intended for the quantitative determination of total PAI-1 in biological fluids. For research use only. Strip well format. Reagents for up to 96 tests.
Rat PAI-1 present in samples reacts with the capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, biotin labeled primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat PAI-1. The amount of color development is directly proportional to the concentration of total PAI-1 in the sample.
More Details:
- Detection Target: PAI-1
- Reactivity: Rat
- Range: 0.05-50 ng/ml
- Sensitivity: 0.022ng/ml
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Please note – this product replaces our old product number IRPAIKT-TOT (same product, new number).